More and more biomedical related research focuses on antibodies, but the premise of related experimental research needs to ensure the purity of antibodies. Based on the most widely used affinity chromatography method, CD BioSciences constructs targeting peptidomimetic ligands of various specific antibodies, which can realize one-step purification, and the types of purified antibodies are not limited.
Overview of Antibody Purification
Since the discovery of antibodies, there have been various methods for the isolation and purification of antibodies. Scientists around the world have been exploring efficient methods for the isolation and purification of antibodies, as well as processes suitable for the large-scale production of antibody drugs. The preparation of highly specific and high-titer antibodies is the basis for immunological research and development of antibody drugs. Unpurified or poorly purified antibodies will directly affect the accuracy, stability and even the success or failure of experimental data.
Fig. 1 Structure of a typical immunoglobulin, IgG. (Arora, et al., 2017)
How to Purify Antibodies?
Polyclonal antibodies, monoclonal antibodies (mAbs) and antibody fragments are usually purified by affinity chromatography, that is, the antibodies and antibody fragments are captured on a carrier containing ligands (e.g., protein A, protein G). The binding of the ligand is reversible, and the target antibody is usually collected by elution by lowering the pH. This method is highly selective, with purity levels above 95%, and purification in one step.
Fig. 2 Steps involved in antibody purification using affinity chromatography. (Ayyar, et al., 2012)
Our Services
CD BioSciences uses the advanced peptidomimetic development platform PepDomTM to design peptidomimetic ligands for specific antibodies, and develop peptidomimetic reagents for purifying related antibodies based on the principle of immunoaffinity.
★ Antibody Types: IgG, IgM, IgD, IgA, IgE
★ Species of Antibody: Humans, Mice, Rats, Dogs, Rhesus Monkeys, Chickens, Rabbits, Camels, etc.
★ Purification Scale: μg-kg
Experimental Rationale
The peptidomimetic ligand developed by CD BioSciences is coupled to the corresponding medium, and the antibody to be purified is added to specifically bind to the peptidomimetic ligand on the medium, while other hybrid proteins and hybrid antibodies are not bound. A series of experimental steps can obtain high-purity target antibodies.
Fig. 3 Schematic diagram of peptidomimetic affinity purification.
The use method of the peptidomimetic purification reagent developed by CD BioSciences is basically the same as the purification method based on protein A/G, only need to replace the ligand protein A/G with the corresponding specific peptidomimetic ligand.
Features of Our Services
- Develop peptidomimetic purification reagents for various types of antibodies according to customer needs.
- Reagent development is carried out by experienced technicians to ensure the highest quality and efficacy.
- Complete and specific quality report, including the sequence, structure of the peptidomimetic, and the result verification report.
- Tailor-made experimental plans for each customer to meet the special needs of each customer.
Based on expertise in immunoaffinity chromatography, CD BioSciences is dedicated to developing purification reagents for multiple antibody types using our unique peptidomimetic development technology. If you are interested in our services, please contact us for the best solution.
References
- Arora, S., Saxena, V., & Ayyar, B. V. (2017). Affinity chromatography: A versatile technique for antibody purification. Methods, 116, 84–94.
- Zarrineh, M., Mashhadi, I. S., Farhadpour, M., & Ghassempour, A. (2020). Mechanism of Antibodies Purification by Protein A. Analytical Biochemistry, 113909.
- Ayyar, B. V., Arora, S., Murphy, C., & O’Kennedy, R. (2012). Affinity chromatography as a tool for antibody purification. Methods, 56(2), 116-129.
All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
